OPRM1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger [TAS]
- behavioral response to ethanol [IMP]
- cellular response to morphine [IBA]
- cellular response to stress [IMP]
- negative regulation of Wnt protein secretion [IMP]
- negative regulation of adenylate cyclase activity [ISS]
- negative regulation of cAMP-mediated signaling [IDA]
- negative regulation of cell proliferation [TAS]
- negative regulation of cytosolic calcium ion concentration [IDA]
- negative regulation of nitric oxide biosynthetic process [IDA]
- neuropeptide signaling pathway [IMP]
- phospholipase C-activating G-protein coupled receptor signaling pathway [ISS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of cAMP-mediated signaling [IDA]
- positive regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of neurogenesis [ISS]
- positive regulation of nitric oxide biosynthetic process [IDA]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISS]
- sensory perception [NAS]
- sensory perception of pain [IBA, ISS]
- synaptic transmission [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ZWILCH
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- kinetochore [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.931388056 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.931388056, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| OPRM1 ZWILCH | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9928 | BioGRID | 2239613 |
Curated By
- BioGRID