BAIT
PBXIP1
HPIP, RP11-307C12.2
pre-B-cell leukemia homeobox interacting protein 1
GO Process (3)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
SLC35B2
PAPST1, SLL, UGTrel4, RP1-302G2.3
solute carrier family 35 (adenosine 3'-phospho 5'-phosphosulfate transporter), member B2
GO Process (11)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- 3'-phospho-5'-adenylyl sulfate transmembrane transport [IDA]
- 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process [TAS]
- 3'-phosphoadenosine 5'-phosphosulfate metabolic process [TAS]
- 3'-phosphoadenosine 5'-phosphosulfate transport [IDA]
- carbohydrate metabolic process [TAS]
- glycosaminoglycan metabolic process [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- signal transduction [IMP]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.763234096 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.763234096, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID