BAIT
AFG3L2
SCA28, SPAX5
AFG3-like AAA ATPase 2
GO Process (0)
GO Function (2)
GO Component (1)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TBX2
T-box 2
GO Process (19)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
- aorta morphogenesis [ISS]
- atrioventricular canal development [ISS]
- cardiac muscle tissue development [ISS]
- cell aging [IDA]
- cellular senescence [IDA]
- embryonic camera-type eye morphogenesis [ISS]
- embryonic digit morphogenesis [ISS]
- endocardial cushion morphogenesis [ISS]
- muscle cell fate determination [ISS]
- negative regulation of cardiac chamber formation [ISS]
- negative regulation of heart looping [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [ISS]
- outflow tract septum morphogenesis [ISS]
- pharynx development [ISS]
- positive regulation of cardiac muscle cell proliferation [ISS]
- positive regulation of cell proliferation [IDA]
- regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [ISS]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999999952 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.999999952, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID