BAIT

KCNK1

DPK, HOHO, K2P1, K2p1.1, KCNO1, TWIK-1, TWIK1, RP4-550F15.1
potassium channel, subfamily K, member 1
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

KCNK1

DPK, HOHO, K2P1, K2p1.1, KCNO1, TWIK-1, TWIK1, RP4-550F15.1
potassium channel, subfamily K, member 1
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Dimerization of TWIK-1 K+ channel subunits via a disulfide bridge.

Lesage F, Reyes R, Fink M, Duprat F, Guillemare E, Lazdunski M

TWIK-1 is a new type of K+ channel with two P domains and is abundantly expressed in human heart and brain. Here we show that TWIK-1 subunits can self-associate to give dimers containing an interchain disulfide bridge. This assembly involves a 34 amino acid domain that is localized to the extracellular M1P1 linker loop. Cysteine 69 which is part of ... [more]

EMBO J. Dec. 02, 1996; 15(23);6400-7 [Pubmed: 8978667]

Throughput

  • Low Throughput

Curated By

  • BioGRID