BAIT
MECOM
AML1-EVI-1, EVI1, MDS1, MDS1-EVI1, PRDM3, hCG_1640438
MDS1 and EVI1 complex locus
GO Process (8)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- hematopoietic stem cell proliferation [ISS]
- negative regulation of JNK cascade [IMP]
- negative regulation of programmed cell death [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of cell cycle [IDA]
- regulation of transcription, DNA-templated [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATP2A1
ATP2A, SERCA1
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
GO Process (18)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- apoptotic mitochondrial changes [IMP]
- blood coagulation [TAS]
- calcium ion import [IMP]
- calcium ion transmembrane transport [IDA]
- calcium ion transport [IDA, IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- ion transmembrane transport [TAS]
- maintenance of mitochondrion location [IMP]
- negative regulation of endoplasmic reticulum calcium ion concentration [IMP]
- negative regulation of striated muscle contraction [IMP]
- positive regulation of endoplasmic reticulum calcium ion concentration [IMP]
- positive regulation of fast-twitch skeletal muscle fiber contraction [IDA]
- positive regulation of mitochondrial calcium ion concentration [IMP]
- regulation of striated muscle contraction [IMP]
- relaxation of skeletal muscle [IDA]
- response to endoplasmic reticulum stress [IMP]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- H zone [IDA]
- I band [IDA]
- calcium channel complex [IC]
- endoplasmic reticulum membrane [IDA, TAS]
- endoplasmic reticulum-Golgi intermediate compartment [ISS]
- integral component of membrane [NAS]
- membrane [ISS]
- perinuclear region of cytoplasm [ISS]
- platelet dense tubular network membrane [TAS]
- sarcoplasmic reticulum [ISS, NAS]
- sarcoplasmic reticulum membrane [IC, TAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.936473303 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.936473303, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID