APLF
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MSH3
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- DNA repair [IDA]
- maintenance of DNA repeat elements [IMP]
- meiotic mismatch repair [IBA]
- mismatch repair [IMP]
- negative regulation of DNA recombination [IDA]
- positive regulation of helicase activity [IDA]
- reciprocal meiotic recombination [IBA]
- somatic recombination of immunoglobulin gene segments [IBA]
Gene Ontology Molecular Function- DNA-dependent ATPase activity [IBA]
- Y-form DNA binding [IBA]
- dinucleotide insertion or deletion binding [IDA]
- dinucleotide repeat insertion binding [IDA]
- double-strand/single-strand DNA junction binding [IBA]
- enzyme binding [IPI]
- guanine/thymine mispair binding [IDA]
- heteroduplex DNA loop binding [IBA]
- mismatched DNA binding [IDA]
- oxidized purine DNA binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- single guanine insertion binding [IDA]
- single-stranded DNA binding [IDA]
- DNA-dependent ATPase activity [IBA]
- Y-form DNA binding [IBA]
- dinucleotide insertion or deletion binding [IDA]
- dinucleotide repeat insertion binding [IDA]
- double-strand/single-strand DNA junction binding [IBA]
- enzyme binding [IPI]
- guanine/thymine mispair binding [IDA]
- heteroduplex DNA loop binding [IBA]
- mismatched DNA binding [IDA]
- oxidized purine DNA binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- single guanine insertion binding [IDA]
- single-stranded DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.974007871 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.974007871, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| APLF MSH3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9974 | BioGRID | 2235398 |
Curated By
- BioGRID