BAIT
GRIN1
GluN1, MRD8, NMD-R1, NMDA1, NMDAR1, NR1, RP11-350O14.1
glutamate receptor, ionotropic, N-methyl D-aspartate 1
GO Process (14)
GO Function (9)
GO Component (14)
Gene Ontology Biological Process
- calcium ion homeostasis [ISS]
- calcium ion transmembrane transport [IDA]
- cation transport [IDA]
- ion transmembrane transport [IBA]
- ionotropic glutamate receptor signaling pathway [IBA, ISS]
- positive regulation of excitatory postsynaptic membrane potential [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- propylene metabolic process [ISS]
- regulation of excitatory postsynaptic membrane potential [ISS]
- regulation of membrane potential [IDA]
- response to ethanol [IDA]
- synaptic transmission [TAS]
- synaptic transmission, glutamatergic [IBA]
- visual learning [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [IDA]
- cell surface [ISS]
- dendrite [IDA]
- dendritic spine [ISS]
- excitatory synapse [ISS]
- integral component of plasma membrane [IDA]
- neuron projection [ISS]
- plasma membrane [TAS]
- postsynaptic density [ISS]
- postsynaptic membrane [IBA, ISS]
- synapse [ISS]
- synaptic cleft [ISS]
- synaptic vesicle [ISS]
- terminal bouton [ISS]
Homo sapiens
PREY
DOCK5
dedicator of cytokinesis 5
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.974605901 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.974605901, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID