BAIT
STRAP
MAWD, PT-WD, UNRIP
serine/threonine kinase receptor associated protein
GO Process (8)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- maintenance of gastrointestinal epithelium [IMP]
- negative regulation of epithelial cell migration [IMP]
- negative regulation of epithelial cell proliferation [IMP]
- negative regulation of epithelial to mesenchymal transition [IMP]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP, TAS]
- spliceosomal snRNP assembly [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RIPK2
CARD3, CARDIAK, CCK, GIG30, RICK, RIP2, WUGSC:H_RG437L15.1
receptor-interacting serine-threonine kinase 2
GO Process (37)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- I-kappaB kinase/NF-kappaB signaling [ISS]
- JNK cascade [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- T cell receptor signaling pathway [ISS, TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- adaptive immune response [ISS]
- cellular response to muramyl dipeptide [IDA]
- inflammatory response [TAS]
- innate immune response [IDA, ISS, TAS]
- negative regulation of apoptotic process [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing 2 signaling pathway [IDA]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- positive regulation of NF-kappaB transcription factor activity [IMP, TAS]
- positive regulation of interferon-alpha production [NAS]
- positive regulation of interferon-beta production [NAS]
- positive regulation of interleukin-12 production [NAS]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of protein ubiquitination [IMP, NAS]
- positive regulation of transcription from RNA polymerase II promoter [NAS]
- signal transduction [TAS]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [IDA, TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.993175729 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.993175729, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID