BAIT
ASC1
ASU9, CPC2, NAD1, guanine nucleotide-binding protein subunit beta, L000003529, L000000135, S000029045, YMR116C
G-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation
GO Process (4)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
DST1
PPR2, SII, S-II, TFIIS, P37, L000001476, L000000530, YGL043W
General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
GO Process (11)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
- mRNA cleavage [IDA, IMP]
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter [IGI, IMP]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA, IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- regulation of mRNA 3'-end processing [IGI, IMP]
- tRNA transcription from RNA polymerase III promoter [IMP]
- transcription antitermination [IDA]
- transcription elongation from RNA polymerase I promoter [IDA]
- transcription elongation from RNA polymerase II promoter [IDA, IMP]
- transcription from RNA polymerase III promoter [IDA]
- transcription initiation from RNA polymerase II promoter [IDA, IGI, IMP]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing.
We used a quantitative, high-density genetic interaction map, or E-MAP (Epistatic MiniArray Profile), to interrogate the relationships within and between RNA-processing pathways. Due to their complexity and the essential roles of many of the components, these pathways have been difficult to functionally dissect. Here, we report the results for 107,155 individual interactions involving 552 mutations, 166 of which are hypomorphic ... [more]
Mol. Cell Dec. 05, 2008; 32(5);735-46 [Pubmed: 19061648]
Quantitative Score
- -4.461344 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (suppression) and S score < -2.5 for negative interactions (synthetic sick/lethality).
Curated By
- BioGRID