BAIT
NOTCH2
AGS2, HJCYS, hN2
notch 2
GO Process (22)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- Notch receptor processing [TAS]
- Notch signaling involved in heart development [IC]
- Notch signaling pathway [TAS]
- apoptotic process [TAS]
- atrial septum morphogenesis [IMP]
- bone remodeling [IMP]
- cell cycle arrest [IDA]
- cell fate determination [TAS]
- cell growth [IDA]
- gene expression [TAS]
- hemopoiesis [TAS]
- intracellular receptor signaling pathway [TAS]
- multicellular organismal development [NAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of cell proliferation [IDA]
- nervous system development [NAS]
- organ morphogenesis [IEP]
- positive regulation of Ras protein signal transduction [IDA]
- pulmonary valve morphogenesis [IMP]
- regulation of transcription, DNA-templated [TAS]
- stem cell maintenance [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
WNT5A
hWNT5A
wingless-type MMTV integration site family, member 5A
GO Process (54)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- Wnt signaling pathway [IDA]
- Wnt signaling pathway, calcium modulating pathway [IDA, IMP]
- activation of JUN kinase activity [IDA, IMP]
- activation of MAPK activity [IDA]
- activation of protein kinase B activity [IDA]
- axon guidance [ISS]
- cell fate commitment [IBA]
- cellular protein localization [IDA]
- cellular response to calcium ion [IEP]
- cellular response to interferon-gamma [IEP]
- cellular response to lipopolysaccharide [IEP]
- cellular response to retinoic acid [ISS]
- cellular response to transforming growth factor beta stimulus [IEP]
- embryonic skeletal system development [IMP]
- epithelial to mesenchymal transition [IEP]
- face development [IMP]
- genitalia development [IMP]
- hematopoietic stem cell proliferation [IDA]
- keratinocyte differentiation [IEP]
- lens development in camera-type eye [ISS]
- male gonad development [IEP]
- negative regulation of apoptotic process [IDA]
- negative regulation of canonical Wnt signaling pathway [IDA, IGI]
- negative regulation of fat cell differentiation [IMP]
- negative regulation of mesenchymal cell proliferation [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neuron differentiation [IBA, ISS]
- olfactory bulb interneuron development [ISS]
- optic cup formation involved in camera-type eye development [ISS]
- palate development [IMP]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of T cell chemotaxis [IMP]
- positive regulation of angiogenesis [IMP]
- positive regulation of cGMP metabolic process [IDA]
- positive regulation of chemokine biosynthetic process [IMP]
- positive regulation of cytokine secretion involved in immune response [IMP]
- positive regulation of endothelial cell migration [IMP]
- positive regulation of endothelial cell proliferation [IMP]
- positive regulation of fibroblast proliferation [IDA]
- positive regulation of inflammatory response [IMP]
- positive regulation of interleukin-1 beta secretion [IMP]
- positive regulation of interleukin-6 production [IMP]
- positive regulation of macrophage activation [IMP]
- positive regulation of macrophage cytokine production [IMP]
- positive regulation of neuron projection development [ISS]
- positive regulation of ossification [IMP]
- positive regulation of protein catabolic process [IGI]
- positive regulation of protein kinase C signaling [IMP]
- positive regulation of response to cytokine stimulus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- positive regulation of type I interferon-mediated signaling pathway [IDA]
- response to organic substance [IEP]
- wound healing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.970502597 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.970502597, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID