NSMF
Gene Ontology Biological Process
- cellular response to amino acid stimulus [ISS]
- cellular response to electrical stimulus [ISS]
- cellular response to gonadotropin stimulus [ISS]
- positive regulation of neuron migration [IMP]
- positive regulation of protein dephosphorylation [ISS]
- regulation of dendrite morphogenesis [ISS]
- regulation of neuron apoptotic process [ISS]
- regulation of neuronal synaptic plasticity [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RBPJ
Gene Ontology Biological Process
- DNA recombination [NAS]
- Notch signaling involved in heart development [IC]
- Notch signaling pathway [IMP, TAS]
- angiogenesis [ISS]
- atrioventricular canal development [ISS]
- blood vessel endothelial cell fate specification [ISS]
- blood vessel lumenization [ISS]
- cardiac left ventricle morphogenesis [ISS]
- dorsal aorta morphogenesis [ISS]
- endocardium morphogenesis [ISS]
- epithelial to mesenchymal transition [ISS]
- epithelial to mesenchymal transition involved in endocardial cushion formation [ISS]
- gene expression [TAS]
- labyrinthine layer blood vessel development [ISS]
- negative regulation of ossification [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [ISS]
- positive regulation of BMP signaling pathway [ISS]
- positive regulation of ERBB signaling pathway [ISS]
- positive regulation of cardiac muscle cell proliferation [ISS]
- positive regulation of cell proliferation involved in heart morphogenesis [ISS]
- positive regulation of ephrin receptor signaling pathway [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription of Notch receptor target [IDA]
- regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation [ISS]
- transcription initiation from RNA polymerase II promoter [TAS]
- ventricular trabecula myocardium morphogenesis [ISS]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- protein binding [IPI]
- recombinase activity [NAS]
- sequence-specific DNA binding transcription factor activity [TAS]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- protein binding [IPI]
- recombinase activity [NAS]
- sequence-specific DNA binding transcription factor activity [TAS]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.999839427 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.999839427, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
NSMF RBPJ | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9998 | BioGRID | 2227696 |
Curated By
- BioGRID