IGF2
Gene Ontology Biological Process
- blood coagulation [TAS]
- cellular protein metabolic process [TAS]
- insulin receptor signaling pathway [TAS]
- insulin receptor signaling pathway via phosphatidylinositol 3-kinase [ISS]
- multicellular organismal development [TAS]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of MAPK cascade [IDA]
- positive regulation of activated T cell proliferation [IDA]
- positive regulation of catalytic activity [ISS]
- positive regulation of cell proliferation [IC]
- positive regulation of glycogen (starch) synthase activity [ISS]
- positive regulation of glycogen biosynthetic process [ISS]
- positive regulation of insulin receptor signaling pathway [IDA]
- positive regulation of mitosis [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [ISS]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of protein phosphorylation [ISS]
- regulation of gene expression by genetic imprinting [TAS]
- regulation of transcription, DNA-templated [NAS]
- skeletal system development [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IGFBP5
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular response to cAMP [IDA]
- cellular response to organic cyclic compound [IDA]
- negative regulation of cell migration [IDA]
- negative regulation of insulin-like growth factor receptor signaling pathway [IDA]
- negative regulation of smooth muscle cell migration [IDA]
- negative regulation of smooth muscle cell proliferation [IDA]
- negative regulation of translation [IDA]
- signal transduction [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.999712022 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.999712022, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IGFBP5 IGF2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3182257 | |
| IGF2 IGFBP5 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID