BAIT
GRB2
ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
growth factor receptor-bound protein 2
GO Process (20)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- Ras protein signal transduction [TAS]
- T cell costimulation [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell-cell signaling [TAS]
- cellular response to ionizing radiation [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IPI, TAS]
- leukocyte migration [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- platelet activation [TAS]
- positive regulation of reactive oxygen species metabolic process [IMP]
- receptor internalization [IMP]
- signal transduction in response to DNA damage [IMP]
Gene Ontology Molecular Function- SH3 domain binding [IDA]
- SH3/SH2 adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [IPI]
- identical protein binding [IPI]
- insulin receptor substrate binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- SH3 domain binding [IDA]
- SH3/SH2 adaptor activity [TAS]
- ephrin receptor binding [IPI]
- epidermal growth factor receptor binding [IPI]
- identical protein binding [IPI]
- insulin receptor substrate binding [IPI]
- neurotrophin TRKA receptor binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
PREY
NKX2-5
CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3
NK2 homeobox 5
GO Process (37)
GO Function (11)
GO Component (3)
Gene Ontology Biological Process
- adult heart development [IMP]
- atrial cardiac muscle cell development [ISS]
- atrial septum morphogenesis [IMP]
- cardiac conduction system development [IMP]
- cardiac muscle cell differentiation [ISS]
- cardiac muscle tissue morphogenesis [IMP]
- cell differentiation [ISS]
- embryonic heart tube development [ISS]
- heart looping [ISS]
- heart morphogenesis [ISS]
- hemopoiesis [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of canonical Wnt signaling pathway [ISS]
- negative regulation of cardiac muscle cell apoptotic process [IMP]
- negative regulation of myotube differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP, ISS]
- negative regulation of transcription, DNA-templated [ISS]
- outflow tract septum morphogenesis [IMP]
- pharyngeal system development [ISS]
- positive regulation of cardioblast differentiation [ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of heart contraction [ISS]
- positive regulation of neuron differentiation [IMP]
- positive regulation of sodium ion transport [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISS]
- positive regulation of transcription initiation from RNA polymerase II promoter [ISS]
- positive regulation of transcription via serum response element binding [ISS]
- positive regulation of transcription, DNA-templated [IDA, ISS]
- positive regulation of voltage-gated calcium channel activity [ISS]
- regulation of cardiac muscle contraction [ISS]
- right ventricular cardiac muscle tissue morphogenesis [IMP]
- septum secundum development [IMP]
- spleen development [IMP, ISS]
- thyroid gland development [IMP]
- vasculogenesis [ISS]
- ventricular cardiac muscle cell development [ISS]
- ventricular septum morphogenesis [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- chromatin binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [ISS]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- serum response element binding [ISS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- chromatin binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [ISS]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- serum response element binding [ISS]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999952833 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.999952833, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GRB2 NKX2-5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2221423 |
Curated By
- BioGRID