BAIT
CDC73
L000002792, YLR418C
Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homologue, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers
GO Process (11)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- mRNA 3'-end processing [IMP]
- negative regulation of DNA recombination [IMP]
- positive regulation of histone H3-K36 trimethylation [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- transcription elongation from RNA polymerase I promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
TUF1
translation elongation factor Tu, tufM, L000002390, YOR187W
Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans
GO Process (2)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing.
We used a quantitative, high-density genetic interaction map, or E-MAP (Epistatic MiniArray Profile), to interrogate the relationships within and between RNA-processing pathways. Due to their complexity and the essential roles of many of the components, these pathways have been difficult to functionally dissect. Here, we report the results for 107,155 individual interactions involving 552 mutations, 166 of which are hypomorphic ... [more]
Mol. Cell Dec. 05, 2008; 32(5);735-46 [Pubmed: 19061648]
Quantitative Score
- -4.664681 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (suppression) and S score < -2.5 for negative interactions (synthetic sick/lethality).
Curated By
- BioGRID