BAIT
HPN
TMPRSS1
hepsin
GO Process (17)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- basement membrane disassembly [IDA]
- cochlea morphogenesis [ISS]
- detection of mechanical stimulus involved in sensory perception of sound [ISS]
- negative regulation of apoptotic process [IDA]
- negative regulation of epithelial cell proliferation [IDA]
- negative regulation of epithelial to mesenchymal transition [IDA]
- pilomotor reflex [ISS]
- positive regulation by host of viral transcription [IDA]
- positive regulation of cell growth [IMP]
- positive regulation of gene expression [ISS]
- positive regulation of hepatocyte proliferation [IDA]
- positive regulation of plasminogen activation [IDA]
- positive regulation of thyroid hormone generation [ISS]
- potassium ion transmembrane transport [ISS]
- proteolysis [IDA]
- regulation of cell shape [IMP]
- response to thyroid hormone [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
LMAN1
ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58
lectin, mannose-binding, 1
GO Process (10)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [TAS]
- Golgi organization [IMP]
- blood coagulation [TAS]
- cellular protein metabolic process [TAS]
- early endosome to Golgi transport [IMP]
- positive regulation of organelle organization [IMP]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- protein exit from endoplasmic reticulum [IMP]
- protein folding [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.886186469 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.886186469, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID