BAIT
YWHAQ
14-3-3, 1C5, HS1
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
GO Process (6)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
F2
PT, RPRGL2, THPH1
coagulation factor II (thrombin)
GO Process (24)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- blood coagulation [TAS]
- blood coagulation, intrinsic pathway [TAS]
- cell surface receptor signaling pathway [IDA]
- cellular protein metabolic process [TAS]
- cytosolic calcium ion homeostasis [IDA]
- fibrinolysis [IDA]
- leukocyte migration [TAS]
- multicellular organismal development [TAS]
- negative regulation of astrocyte differentiation [IDA]
- negative regulation of fibrinolysis [TAS]
- negative regulation of platelet activation [TAS]
- negative regulation of proteolysis [IDA]
- peptidyl-glutamic acid carboxylation [TAS]
- platelet activation [IDA, TAS]
- positive regulation of blood coagulation [IDA]
- positive regulation of collagen biosynthetic process [IDA]
- positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of reactive oxygen species metabolic process [IDA]
- positive regulation of release of sequestered calcium ion into cytosol [IDA]
- post-translational protein modification [TAS]
- proteolysis [TAS]
- regulation of blood coagulation [TAS]
- response to wounding [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.998725571 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.998725571, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID