LSM14B
Gene Ontology Molecular Function
CASP3
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [TAS]
- apoptotic DNA fragmentation [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- erythrocyte differentiation [IDA, TAS]
- execution phase of apoptosis [IDA, IMP]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- hippo signaling [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- keratinocyte differentiation [IBA]
- negative regulation of apoptotic process [IGI]
- neuron differentiation [IBA]
- neurotrophin TRK receptor signaling pathway [TAS]
- platelet formation [TAS]
- positive regulation of apoptotic process [TAS]
- proteolysis [IDA]
- regulation of apoptotic process [TAS]
- regulation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- response to tumor necrosis factor [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [IDA, TAS]
- nucleoplasm [TAS]
- nucleus [IDA]
- plasma membrane [TAS]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.864082207 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.864082207, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LSM14B CASP3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.8173 | BioGRID | 2267126 |
Curated By
- BioGRID