BAIT
DNAJC19
PAM18, TIM14, TIMM14
DnaJ (Hsp40) homolog, subfamily C, member 19
GO Process (5)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GHRL
MTLRP, UNQ524/PRO1066
ghrelin/obestatin prepropeptide
GO Process (36)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS, TAS]
- actin polymerization or depolymerization [IDA]
- activation of MAPK activity [IMP]
- adult feeding behavior [ISS]
- cartilage development [NAS]
- cellular protein metabolic process [TAS]
- cortisol secretion [NAS]
- decidualization [IDA]
- dendrite development [IDA]
- gastric acid secretion [IBA]
- glucose metabolic process [NAS]
- growth hormone secretion [TAS]
- hormone-mediated signaling pathway [TAS]
- negative regulation of angiogenesis [NAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of circadian sleep/wake cycle, REM sleep [IDA]
- negative regulation of endothelial cell proliferation [IDA]
- negative regulation of inflammatory response [IDA]
- negative regulation of interleukin-1 beta production [IDA]
- negative regulation of interleukin-6 biosynthetic process [IDA]
- negative regulation of tumor necrosis factor biosynthetic process [IDA]
- positive regulation of appetite [ISS]
- positive regulation of circadian sleep/wake cycle, non-REM sleep [IDA]
- positive regulation of corticotropin secretion [IDA]
- positive regulation of cortisol secretion [IDA]
- positive regulation of cytosolic calcium ion concentration [ISS]
- positive regulation of growth hormone receptor signaling pathway [IC]
- positive regulation of growth hormone secretion [IDA]
- positive regulation of insulin secretion [ISS]
- positive regulation of multicellular organism growth [IC]
- positive regulation of protein tyrosine kinase activity [IMP]
- positive regulation of synapse assembly [IDA]
- regulation of cell proliferation [IDA]
- regulation of response to food [IBA]
- response to estrogen [IDA]
- response to hormone [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.998117325 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.998117325, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID