BAIT
FBXO2
FBG1, FBX2, Fbs1, NFB42, OCP1
F-box protein 2
GO Process (5)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ROBO1
DUTT1, SAX3
roundabout, axon guidance receptor, homolog 1 (Drosophila)
GO Process (13)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- Roundabout signaling pathway [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- axon guidance [TAS]
- axon midline choice point recognition [ISS]
- cell adhesion [TAS]
- cell migration involved in sprouting angiogenesis [IMP]
- chemorepulsion involved in postnatal olfactory bulb interneuron migration [IDA]
- homophilic cell adhesion via plasma membrane adhesion molecules [IDA]
- negative regulation of chemokine-mediated signaling pathway [IMP]
- negative regulation of mammary gland epithelial cell proliferation [IMP]
- negative regulation of negative chemotaxis [IDA]
- nervous system development [TAS]
- positive regulation of axonogenesis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.920493721 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.920493721, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID