BAIT
EFNA5
AF1, EFL5, EPLG7, GLC1M, LERK7, RAGS
ephrin-A5
GO Process (12)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- apoptotic process [IDA]
- axon guidance [IBA]
- ephrin receptor signaling pathway [IDA]
- nervous system development [TAS]
- positive regulation of peptidyl-tyrosine phosphorylation [ISS]
- regulation of Rac GTPase activity [ISS]
- regulation of Rho GTPase activity [IDA]
- regulation of actin cytoskeleton organization [IDA]
- regulation of cell-cell adhesion [IDA]
- regulation of focal adhesion assembly [IDA]
- regulation of insulin secretion involved in cellular response to glucose stimulus [ISS]
- regulation of microtubule cytoskeleton organization [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
EPHA2
ARCC2, CTPA, CTPP1, CTRCT6, ECK
EPH receptor A2
GO Process (23)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- activation of Rac GTPase activity [IMP]
- bone remodeling [ISS]
- branching involved in mammary gland duct morphogenesis [ISS]
- cell chemotaxis [IMP]
- cell migration [IMP]
- ephrin receptor signaling pathway [IDA]
- intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- keratinocyte differentiation [IMP]
- lens fiber cell morphogenesis [ISS]
- mammary gland epithelial cell proliferation [ISS]
- multicellular organismal development [TAS]
- negative regulation of protein kinase B signaling [IDA]
- osteoblast differentiation [ISS]
- osteoclast differentiation [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- protein kinase B signaling [IDA]
- regulation of ERK1 and ERK2 cascade [IMP]
- regulation of angiogenesis [ISS]
- regulation of blood vessel endothelial cell migration [ISS]
- regulation of cell adhesion mediated by integrin [IDA]
- regulation of lamellipodium assembly [IMP]
- response to growth factor [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.996295042 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.996295042, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID