BAIT
IDH3B
H-IDHB, RP46, RP4-686C3.2
isocitrate dehydrogenase 3 (NAD+) beta
GO Process (3)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CHI3L1
ASRT7, CGP-39, GP-39, GP39, HC-gp39, HCGP-3P, YKL-40, YKL40, YYL-40, hCGP-39
chitinase 3-like 1 (cartilage glycoprotein-39)
GO Process (16)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- activation of NF-kappaB-inducing kinase activity [ISS]
- cartilage development [NAS]
- cellular response to tumor necrosis factor [ISS]
- chitin catabolic process [IBA]
- immune response [IBA]
- inflammatory response [IEP]
- interleukin-8 secretion [ISS]
- lung development [IMP]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of angiogenesis [IMP]
- positive regulation of peptidyl-threonine phosphorylation [ISS]
- positive regulation of protein kinase B signaling [IMP]
- response to interleukin-1 [IEP]
- response to interleukin-6 [IEP]
- response to mechanical stimulus [IEP]
- response to tumor necrosis factor [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.977104665 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.977104665, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID