BAIT
GOT1
ASTQTL1, GIG18, cAspAT, cCAT
glutamic-oxaloacetic transaminase 1, soluble
GO Process (16)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- 2-oxoglutarate metabolic process [ISS]
- L-methionine biosynthetic process from methylthioadenosine [TAS]
- aspartate catabolic process [IDA]
- aspartate metabolic process [ISS]
- carbohydrate metabolic process [TAS]
- cellular amino acid biosynthetic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- cellular response to insulin stimulus [IEP]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glutamate metabolic process [ISS]
- glycerol biosynthetic process [ISS]
- polyamine metabolic process [TAS]
- response to glucocorticoid [IEP]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
RAB14
FBP, RAB-14, RP11-165P4.4
RAB14, member RAS oncogene family
GO Process (12)
GO Function (5)
GO Component (19)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- Golgi to endosome transport [ISS, TAS]
- Rab protein signal transduction [IBA]
- embryo development [ISS]
- endocytic recycling [IDA]
- fibroblast growth factor receptor signaling pathway [ISS]
- intracellular protein transport [IBA]
- intracellular transport [NAS]
- membrane organization [TAS]
- phagolysosome assembly involved in apoptotic cell clearance [IBA]
- regulation of protein localization [IDA]
- vesicle-mediated transport [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IBA]
- Golgi stack [ISS]
- cytoplasmic vesicle membrane [TAS]
- cytosol [ISS]
- early endosome [IBA, ISS]
- extracellular vesicular exosome [IDA]
- intracellular [IDA]
- intracellular membrane-bounded organelle [IDA]
- late endosome [ISS]
- lysosomal membrane [IDA]
- lysosome [ISS]
- nuclear outer membrane-endoplasmic reticulum membrane network [ISS]
- perinuclear region of cytoplasm [ISS]
- phagocytic vesicle [IDA]
- plasma membrane [ISS]
- primary cilium [IDA]
- recycling endosome [IDA]
- rough endoplasmic reticulum [ISS]
- trans-Golgi network transport vesicle [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.934452898 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.934452898, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID