RPL4
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TOP2A
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA ligation [IDA]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IBA]
- apoptotic chromosome condensation [IDA]
- cellular response to DNA damage stimulus [IDA]
- chromosome segregation [IMP]
- mitotic DNA integrity checkpoint [IBA]
- mitotic cell cycle [TAS]
- mitotic recombination [IBA]
- positive regulation of apoptotic process [IDA]
- positive regulation of single stranded viral RNA replication via double stranded DNA intermediate [IMP]
- positive regulation of viral genome replication [IMP]
- resolution of meiotic recombination intermediates [IBA]
- sister chromatid segregation [IBA]
Gene Ontology Molecular Function- DNA binding [IDA]
- DNA binding, bending [IDA]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA]
- DNA-dependent ATPase activity [IDA]
- chromatin binding [IDA]
- drug binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- magnesium ion binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- protein kinase C binding [IPI]
- ubiquitin binding [IMP]
- DNA binding [IDA]
- DNA binding, bending [IDA]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA]
- DNA-dependent ATPase activity [IDA]
- chromatin binding [IDA]
- drug binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- magnesium ion binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- protein kinase C binding [IPI]
- ubiquitin binding [IMP]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.978023393 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.978023393, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TOP2A RPL4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3373769 | |
| RPL4 TOP2A | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | 3756975 |
Curated By
- BioGRID