Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Defining of the minimal domain of protein 4.1 involved in spectrin-actin binding.

Schischmanoff PO, Winardi R, Discher DE, Parra MK, Bicknese SE, Witkowska HE, Conboy JG, Mohandas N

The spectrin-actin-binding domain of protein 4.1 is encoded by a 21-amino acid alternative exon and a 59-amino acid constitutive exon. To characterize the minimal domain active for interactions with spectrin and actin, we functionally characterized recombinant 4.1 peptides containing the 21-amino acid cassette plus varying portions of the 59-amino acid cassette (designated 21.10 to 21.59). Peptide 21.43 was shown fully ... [more]

J. Biol. Chem. Sep. 08, 1995; 270(36);21243-50 [Pubmed: 7673158]

Throughput

  • Low Throughput

Curated By

  • BioGRID