BAIT

NFKBIA

IKBA, MAD-3, NFKBI
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
GO Process (31)
GO Function (7)
GO Component (5)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The PEST domain of IkappaBalpha is necessary and sufficient for in vitro degradation by mu-calpain.

Shumway SD, Maki M, Miyamoto S

Polypeptide sequences enriched in proline (P), glutamate (E), serine (S), and threonine (T), dubbed PEST domains, are proposed to expedite the degradation of proteins. The proteolysis of one PEST-containing protein, IkappaBalpha, is prerequisite to the activation of the transcription factor NF-kappaB. Two mechanisms of IkappaBalpha degradation in vivo have been described, one well characterized through the ubiquitin-proteasome pathway, and another ... [more]

J. Biol. Chem. Oct. 22, 1999; 274(43);30874-81 [Pubmed: 10521480]

Throughput

  • Low Throughput

Curated By

  • BioGRID