AXIN2
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IBA]
- cellular protein localization [IDA]
- cellular response to organic cyclic compound [IBA]
- dorsal/ventral axis specification [IBA]
- intramembranous ossification [IBA]
- mRNA stabilization [IMP]
- maintenance of DNA repeat elements [IMP]
- negative regulation of canonical Wnt signaling pathway [IDA, IMP]
- negative regulation of catenin import into nucleus [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of osteoblast differentiation [IBA]
- odontogenesis [IMP]
- positive regulation of GTPase activity [IBA]
- positive regulation of cell death [IMP]
- positive regulation of epithelial to mesenchymal transition [IMP]
- positive regulation of protein phosphorylation [IMP]
- regulation of centromeric sister chromatid cohesion [IMP]
- regulation of mismatch repair [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
APC
Gene Ontology Biological Process
- apoptotic process [TAS]
- canonical Wnt signaling pathway [IC, NAS]
- cell adhesion [NAS]
- cell cycle arrest [IDA]
- cell migration [IMP]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cellular response to DNA damage stimulus [IDA]
- mitotic cytokinesis [IMP]
- mitotic spindle assembly checkpoint [IMP]
- negative regulation of canonical Wnt signaling pathway [IGI]
- negative regulation of cell proliferation [IDA]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of microtubule depolymerization [IDA, IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of protein catabolic process [IC, IGI]
- positive regulation of pseudopodium assembly [IMP]
- protein complex assembly [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP, NAS]
- regulation of microtubule-based process [IMP]
- tight junction assembly [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Hot spots in beta-catenin for interactions with LEF-1, conductin and APC.
Interactions between beta-catenin and LEF-1/TCF, APC and conductin/axin are essential for wnt-controlled stabilization of beta-catenin and transcriptional activation. The wnt signal transduction pathway is important in both embryonic development and tumor progression. We identify here amino acid residues in beta-catenin that distinctly affect its binding to LEF-1/TCF, APC and conductin. These residues form separate surface clusters, termed hot spots, along ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID