An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death.

Kelly K, Manos E, Jensen G, Nadauld L, Jones DA

We have examined the activity of a new member of the tumor necrosis factor (TNF) family identified through Expressed Sequence Tag database searching using TNFalpha protein as the search query. We have termed this protein TNF-related death ligand-la (TRDL-1alpha). Traditional cDNA library screening identified two additional splice variants designated TRDL-1beta and TRDL-1gamma that differed from TRDL-1alpha by the deletion of ... [more]

Cancer Res. Feb. 15, 2000; 60(4);1021-7 [Pubmed: 10706119]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TNFSF13

Gene Ontology Biological Process

Gene Ontology Molecular Function

receptor binding [TAS]

Gene Ontology Cellular Component

TNFRSF14