Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Crystal structure of the pleckstrin homology domain from dynamin.

Timm D, Salim K, Gout I, Guruprasad L, Waterfield M, Blundell T

The pleckstrin homology (PH) domain is a conserved module present in many signal transducing and cytoskeletal proteins. Here we report the 2.8 A crystal structure of the PH domain from dynamin. This domain consists of seven beta-strands forming two roughly orthogonal antiparallel beta-sheets terminating with an amphipathic alpha-helix. The structure also reveals a non-covalent dimeric association of the PH domain ... [more]

Nat. Struct. Biol. Nov. 01, 1994; 1(11);782-8 [Pubmed: 7634088]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DNM1 DNM1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Mishra RK (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DNM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [ISS]

Gene Ontology Cellular Component