BAIT

MAML2

MAM-3, MAM2, MAM3, MLL-MAML2
mastermind-like 2 (Drosophila)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors.

Wu L, Sun T, Kobayashi K, Gao P, Griffin JD

The molecular mechanisms by which Notch receptors induce diverse biological responses are not fully understood. We recently cloned a mammalian homologue of the Mastermind gene of Drosophila melanogaster, MAML1 (Mastermind-like-1 molecule) and determined that it functions as a transcriptional coactivator for Notch receptors. In this report, we characterize two additional genes in this Mastermind-like gene family: MAML2 and MAML3. The ... [more]

Mol. Cell. Biol. Nov. 01, 2002; 22(21);7688-700 [Pubmed: 12370315]

Throughput

  • Low Throughput

Curated By

  • BioGRID