BAIT

TPH1

TPRH, TRPH
tryptophan hydroxylase 1
GO Process (3)
GO Function (0)
GO Component (1)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Demonstration of the phosphorylation-dependent interaction of tryptophan hydroxylase with the 14-3-3 protein.

Furukawa Y, Ikuta N, Omata S, Yamauchi T, Isobe T, Ichimura T

The molecular mechanism of the phosphorylation-dependent activation of tryptophan hydroxylase is studied with respect to the role of the 14-3-3 protein. Reexamination of the system reconstituted with the purified TRH and the 14-3-3 protein showed that the level of the TRH activity correlated with the extent of the Ca2+/calmodulin- or the cAMP-dependent phosphorylation in TRH. The experiment confirmed the requirement ... [more]

Biochem. Biophys. Res. Commun. Jul. 15, 1993; 194(1);144-9 [Pubmed: 8101440]

Throughput

  • Low Throughput

Curated By

  • BioGRID