BAIT
SLC1A1
DCBXA, EAAC1, EAAT3, SCZD18, RP11-6J24.1
solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
GO Process (7)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SORT1
Gp95, LDLCQ6, NT3
sortilin 1
GO Process (16)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IMP]
- Golgi to endosome transport [IDA]
- endocytosis [IDA]
- endosome to lysosome transport [IDA]
- endosome transport via multivesicular body sorting pathway [IDA]
- extrinsic apoptotic signaling pathway via death domain receptors [IDA]
- glucose import [IMP]
- myotube differentiation [IMP]
- negative regulation of lipoprotein lipase activity [IDA]
- nerve growth factor signaling pathway [IDA]
- neuropeptide signaling pathway [IDA]
- neurotrophin TRK receptor signaling pathway [IMP]
- plasma membrane to endosome transport [IDA]
- regulation of gene expression [IMP]
- response to insulin [IMP]
- vesicle organization [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.954564366 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.954564366, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID