BAIT
IFNG
IFG, IFI
interferon, gamma
GO Process (32)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
- adaptive immune response [IBA]
- apoptotic process [IGI]
- cell cycle arrest [IDA]
- cell surface receptor signaling pathway [TAS]
- cellular component movement [TAS]
- cytokine-mediated signaling pathway [TAS]
- extrinsic apoptotic signaling pathway [IDA]
- humoral immune response [IBA]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of epithelial cell differentiation [ISS]
- negative regulation of interleukin-17 production [IDA]
- negative regulation of smooth muscle cell proliferation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of calcidiol 1-monooxygenase activity [IDA]
- positive regulation of cell proliferation [ISS]
- positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity [IDA]
- positive regulation of fructose 1,6-bisphosphate metabolic process [IDA]
- positive regulation of interleukin-12 production [IDA]
- positive regulation of interleukin-23 production [IDA]
- positive regulation of killing of cells of other organism [IDA]
- positive regulation of membrane protein ectodomain proteolysis [IDA]
- positive regulation of nitric oxide biosynthetic process [IDA]
- positive regulation of osteoclast differentiation [IDA]
- positive regulation of peptidyl-serine phosphorylation of STAT protein [IDA, NAS]
- positive regulation of smooth muscle cell apoptotic process [IDA]
- positive regulation of tumor necrosis factor (ligand) superfamily member 11 production [IDA]
- positive regulation of tyrosine phosphorylation of Stat1 protein [IDA]
- positive regulation of vitamin D biosynthetic process [IDA]
- protein import into nucleus, translocation [IDA]
- regulation of insulin secretion [IDA]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
- response to virus [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NEDD4
NEDD4-1, RPF1, PIG53
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
GO Process (26)
GO Function (10)
GO Component (11)
Gene Ontology Biological Process
- cellular response to UV [IMP]
- cytokine-mediated signaling pathway [TAS]
- development involved in symbiotic interaction [IMP]
- glucocorticoid receptor signaling pathway [IDA]
- lysosomal transport [IDA]
- negative regulation of sodium ion transport [IDA]
- negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [IMP]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISS]
- neuron projection development [IEP]
- positive regulation of nucleocytoplasmic transport [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of protein catabolic process [IDA]
- progesterone receptor signaling pathway [IDA]
- protein K63-linked ubiquitination [ISS]
- protein targeting to lysosome [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, IMP]
- receptor catabolic process [IDA]
- receptor internalization [IDA]
- regulation of dendrite morphogenesis [ISS]
- regulation of ion transmembrane transport [IDA]
- regulation of membrane potential [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- response to calcium ion [TAS]
- transmission of virus [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.841865546 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.841865546, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID