An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Fibulin binds to itself and to the carboxyl-terminal heparin-binding region of fibronectin.

Balbona K, Tran H, Godyna S, Ingham KC, Strickland DK, Argraves WS

Fibulin is a recently described extracellular matrix (ECM) and plasma glycoprotein (Argraves, W. S., Tran, H., Burgess, W. H., and Dickerson, K. (1990) J. Cell Biol. 111, 3155-3164). In this report, ligand affinity chromatography and solid-phase binding analyses were performed to determine which ECM protein(s) interact with fibulin. Fibulin-Sepharose bound two polypeptides of 240 and 100 kDa from the culture ... [more]

J. Biol. Chem. Oct. 05, 1992; 267(28);20120-5 [Pubmed: 1400330]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FBLN1 FN1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wang J (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

collagen binding [NAS]heparin binding [NAS]integrin binding [IDA, IMP]protease binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

FBLN1