BAIT

CD36

BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3
CD36 molecule (thrombospondin receptor)
GO Process (27)
GO Function (6)
GO Component (8)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CD36 associates with CD9 and integrins on human blood platelets.

Miao WM, Vasile E, Lane WS, Lawler J

The membrane glycoprotein CD36 is involved in platelet aggregation, inhibition of angiogenesis, atherosclerosis, and sequestration of malaria-parasitized erythrocytes. In this study, immunoprecipitations with anti-CD36 antibodies were performed to identify proteins that associate with CD36 in the platelet membrane. Platelets were solubilized in 1% Triton X-100, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), Brij 96, or Brij 99, and the proteins that coprecipitated with CD36 were ... [more]

Blood Mar. 15, 2001; 97(6);1689-96 [Pubmed: 11238109]

Throughput

  • Low Throughput

Curated By

  • BioGRID