Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution.

Choi HJ, Kang SW, Yang CH, Rhee SG, Ryu SE

Hydrogen peroxide (H2O2) has been implicated recently as an intracellular messenger that affects cellular processes including protein phosphorylation, transcription and apoptosis. A set of novel peroxidases, named peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the presence of an appropriate electron donor. The crystal structure of a human Prx enzyme, hORF6, reveals that the protein contains ... [more]

Nat. Struct. Biol. May. 01, 1998; 5(5);400-6 [Pubmed: 9587003]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRDX6 PRDX6	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Ralat LA (2006)	Low	-	BioGRID	1534040
PRDX6 PRDX6	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Wang J (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PRDX6

Gene Ontology Biological Process

Gene Ontology Molecular Function

antioxidant activity [NAS]phospholipase A2 activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component