BAIT
DCTN3
DCTN-22, DCTN22, RP11-296L22.3
dynactin 3 (p22)
GO Process (4)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
THBS1
THBS, THBS-1, TSP, TSP-1, TSP1
thrombospondin 1
GO Process (60)
GO Function (15)
GO Component (12)
Gene Ontology Biological Process
- activation of MAPK activity [IMP]
- behavioral response to pain [ISS]
- blood coagulation [TAS]
- cell adhesion [NAS]
- cell cycle arrest [IDA]
- cell migration [IDA]
- cellular response to heat [NAS]
- chronic inflammatory response [IEP]
- engulfment of apoptotic cell [IDA]
- extracellular matrix organization [TAS]
- immune response [IEP]
- negative regulation of angiogenesis [IDA]
- negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II [IDA]
- negative regulation of apoptotic process [IDA]
- negative regulation of blood vessel endothelial cell migration [IDA]
- negative regulation of cGMP-mediated signaling [IDA]
- negative regulation of cell-matrix adhesion [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of dendritic cell antigen processing and presentation [IDA]
- negative regulation of endothelial cell chemotaxis [IDA]
- negative regulation of endothelial cell migration [IDA]
- negative regulation of endothelial cell proliferation [IDA, IMP]
- negative regulation of extrinsic apoptotic signaling pathway [TAS]
- negative regulation of fibrinolysis [IDA]
- negative regulation of fibroblast growth factor receptor signaling pathway [IDA]
- negative regulation of focal adhesion assembly [TAS]
- negative regulation of interleukin-12 production [IDA]
- negative regulation of nitric oxide mediated signal transduction [IDA]
- negative regulation of plasma membrane long-chain fatty acid transport [IDA]
- negative regulation of plasminogen activation [IDA]
- peptide cross-linking [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of angiogenesis [IMP]
- positive regulation of blood coagulation [IDA]
- positive regulation of blood vessel endothelial cell migration [IDA]
- positive regulation of cell migration [IDA, IMP]
- positive regulation of chemotaxis [IDA]
- positive regulation of endothelial cell apoptotic process [IDA]
- positive regulation of endothelial cell migration [IDA]
- positive regulation of extrinsic apoptotic signaling pathway via death domain receptors [IDA]
- positive regulation of fibroblast migration [IDA]
- positive regulation of macrophage activation [IDA]
- positive regulation of macrophage chemotaxis [ISS]
- positive regulation of phosphorylation [IMP]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of reactive oxygen species metabolic process [IDA]
- positive regulation of transforming growth factor beta receptor signaling pathway [IDA]
- positive regulation of transforming growth factor beta1 production [ISS]
- positive regulation of translation [IDA]
- positive regulation of tumor necrosis factor biosynthetic process [IDA]
- regulation of cGMP metabolic process [IDA]
- response to calcium ion [IDA]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISS]
- response to glucose [IDA]
- response to hypoxia [NAS]
- response to magnesium ion [IDA]
- response to progesterone [TAS]
- sprouting angiogenesis [IMP]
Gene Ontology Molecular Function- calcium ion binding [NAS]
- collagen V binding [IDA]
- fibrinogen binding [IDA]
- fibroblast growth factor binding [IDA]
- fibronectin binding [IDA]
- glycoprotein binding [NAS]
- heparin binding [IDA]
- identical protein binding [NAS]
- integrin binding [IMP]
- laminin binding [IDA]
- low-density lipoprotein particle binding [IDA]
- phosphatidylserine binding [IDA]
- protein binding [IPI]
- proteoglycan binding [TAS]
- transforming growth factor beta binding [ISS, TAS]
- calcium ion binding [NAS]
- collagen V binding [IDA]
- fibrinogen binding [IDA]
- fibroblast growth factor binding [IDA]
- fibronectin binding [IDA]
- glycoprotein binding [NAS]
- heparin binding [IDA]
- identical protein binding [NAS]
- integrin binding [IMP]
- laminin binding [IDA]
- low-density lipoprotein particle binding [IDA]
- phosphatidylserine binding [IDA]
- protein binding [IPI]
- proteoglycan binding [TAS]
- transforming growth factor beta binding [ISS, TAS]
Gene Ontology Cellular Component
- cell surface [IDA]
- endoplasmic reticulum [ISS]
- external side of plasma membrane [IDA]
- extracellular matrix [IDA, ISS, TAS]
- extracellular region [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- fibrinogen complex [IDA]
- platelet alpha granule [IDA]
- platelet alpha granule lumen [TAS]
- sarcoplasmic reticulum [ISS]
- secretory granule [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.960825968 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.960825968, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID