BAIT

KCNJ4

HIR, HIRK2, HRK1, IRK-3, IRK3, Kir2.3, RP3-434P1.2
potassium inwardly-rectifying channel, subfamily J, member 4
GO Process (4)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

GNGT2

G-GAMMA-8, G-GAMMA-C, GNG8, GNG9, GNGT8
guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 2
GO Process (4)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Inhibition of an inward rectifier potassium channel (Kir2.3) by G-protein betagamma subunits.

Cohen NA, Sha Q, Makhina EN, Lopatin AN, Linder ME, Snyder SH, Nichols CG

The molecular basis of G-protein inhibition of inward rectifier K+ currents was examined by co-expression of G-proteins and cloned Kir2 channel subunits in Xenopus oocytes. Channels encoded by Kir2.3 (HRK1/HIR/BIRK2/BIR11) were completely suppressed by co-expression with G-protein betagamma subunits, whereas channels encoded by Kir2. 1 (IRK1), which shares 60% amino acid identity with Kir2.3, were unaffected. Co-expression of Galphai1 and ... [more]

J. Biol. Chem. Dec. 13, 1996; 271(50);32301-5 [Pubmed: 8943291]

Throughput

  • Low Throughput

Curated By

  • BioGRID