BAIT
METTL21B
FAM119B
methyltransferase like 21B
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
RPS6KA5
MSK1, MSPK1, RLPK
ribosomal protein S6 kinase, 90kDa, polypeptide 5
GO Process (32)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- axon guidance [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- histone H2A-S1 phosphorylation [IDA]
- histone H3-S10 phosphorylation [IMP]
- histone H3-S28 phosphorylation [IMP]
- histone phosphorylation [IDA, TAS]
- innate immune response [TAS]
- interleukin-1-mediated signaling pathway [IMP]
- intracellular signal transduction [IDA]
- negative regulation of cytokine production [TAS]
- negative regulation of transcription, DNA-templated [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of CREB transcription factor activity [TAS]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of histone acetylation [IMP]
- positive regulation of histone phosphorylation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein phosphorylation [IDA]
- regulation of transcription, DNA-templated [IDA, IMP]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999959421 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.999959421, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID