BAIT

MASP1

3MC1, CRARF, CRARF1, MAP1, MASP, MASP3, MAp44, PRSS5, RaRF
mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
Homo sapiens
PREY

FCN2

EBP-37, FCNL, P35, ficolin-2, RP11-263F14.2
ficolin (collagen/fibrinogen domain containing lectin) 2
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Characterization of the interaction between L-ficolin/p35 and mannan-binding lectin-associated serine proteases-1 and -2.

Cseh S, Vera L, Matsushita M, Fujita T, Arlaud GJ, Thielens NM

Ficolins are oligomeric lectins comprising a collagen-like and a fibrinogen-like domain, with a binding specificity for N-acetylglucosamine. It has been reported recently that L-ficolin/P35 associates with mannan-binding lectin (MBL)-associated serine proteases (MASP-1 and -2) and MBL-associated protein 19 (MAp19) in serum and forms complexes able to activate complement. Using surface plasmon resonance spectroscopy we have shown that recombinant MASP-1 and ... [more]

J. Immunol. Nov. 15, 2002; 169(10);5735-43 [Pubmed: 12421953]

Throughput

  • Low Throughput

Curated By

  • BioGRID