BAIT

NEDD4

AA959633, AL023035, AU019897, E430025J12Rik, Nedd4-1, Nedd4a, mKIAA0093
neural precursor cell expressed, developmentally down-regulated 4
Mus musculus
PREY

N4BP3

LZTS4
NEDD4 binding protein 3
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase.

Murillas R, Simms KS, Hatakeyama S, Weissman AM, Kuehn MR

Nedd4 is a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins. The molecular basis for the interaction of Nedd4 with substrates lies in its WW domains, which can bind proline-rich (PY) domains in target proteins. Nedd4 is a developmentally expressed protein and may have a fundamental role to play in embryonic processes. However, whether Nedd4 ... [more]

J. Biol. Chem. Jan. 25, 2002; 277(4);2897-907 [Pubmed: 11717310]

Throughput

  • Low Throughput

Curated By

  • BioGRID