An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Uridine phosphorylase association with vimentin. Intracellular distribution and localization.

Russell RL, Cao D, Zhang D, Handschumacher RE, Pizzorno G

Uridine phosphorylase (UPase), a key enzyme in the pyrimidine salvage pathway, is associated with the intermediate filament protein vimentin, in NIH 3T3 fibroblasts and colon 26 cells. Affinity chromatography was utilized to purify UPase from colon 26 and NIH 3T3 cells using the uridine phosphorylase inhibitor 5'-amino benzylacyclouridine linked to an agarose matrix. Vimentin copurification with UPase was confirmed using ... [more]

J. Biol. Chem. Apr. 20, 2001; 276(16);13302-7 [Pubmed: 11278417]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UPP2 VIM	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Russell RL (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

UPP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

uridine phosphorylase activity [IDA]

Gene Ontology Cellular Component

VIM