BAIT
PLEK
P47
pleckstrin
GO Process (25)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- actin cytoskeleton reorganization [IDA]
- blood coagulation [TAS]
- cell projection organization [IDA]
- cortical actin cytoskeleton organization [IDA]
- hematopoietic progenitor cell differentiation [IEP]
- integrin-mediated signaling pathway [IDA]
- negative regulation of G-protein coupled receptor protein signaling pathway [IDA]
- negative regulation of calcium-mediated signaling [NAS]
- negative regulation of inositol phosphate biosynthetic process [IDA]
- phosphatidylinositol metabolic process [IDA]
- phospholipase C-inhibiting G-protein coupled receptor signaling pathway [IDA]
- platelet activation [TAS]
- platelet aggregation [IMP, ISS]
- platelet degranulation [IEP, TAS]
- positive regulation of actin filament bundle assembly [IDA]
- positive regulation of actin filament depolymerization [IDA]
- positive regulation of inositol-polyphosphate 5-phosphatase activity [IDA]
- positive regulation of integrin activation [ISS]
- positive regulation of platelet activation [ISS]
- protein kinase C signaling [ISS]
- protein secretion by platelet [ISS]
- regulation of cell diameter [IDA]
- ruffle organization [IDA]
- thrombin receptor signaling pathway [IDA]
- vesicle docking involved in exocytosis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ARHGEF1
GEF1, LBCL2, LSC, P115-RHOGEF, SUB1.5
Rho guanine nucleotide exchange factor (GEF) 1
GO Process (6)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.799149652 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.799149652, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID