BAIT
ACADL
ACAD4, LCAD
acyl-CoA dehydrogenase, long chain
GO Process (12)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- carnitine catabolic process [ISS]
- carnitine metabolic process, CoA-linked [ISS]
- cellular lipid catabolic process [ISS]
- cellular lipid metabolic process [TAS]
- fatty acid beta-oxidation [TAS]
- fatty acid beta-oxidation using acyl-CoA dehydrogenase [ISS]
- negative regulation of fatty acid biosynthetic process [ISS]
- negative regulation of fatty acid oxidation [ISS]
- oxidation-reduction process [ISS]
- regulation of cholesterol metabolic process [ISS]
- small molecule metabolic process [TAS]
- temperature homeostasis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAP1B
K-REV, RAL1B, OK/SW-cl.11
RAP1B, member of RAS oncogene family
GO Process (10)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- Rap protein signal transduction [IMP]
- blood coagulation [TAS]
- cellular response to cAMP [IDA]
- energy reserve metabolic process [TAS]
- establishment of endothelial barrier [IMP]
- platelet activation [TAS]
- regulation of cell junction assembly [IMP]
- regulation of establishment of cell polarity [IMP]
- regulation of insulin secretion [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.853877108 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.853877108, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID