Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Activation of the guanine nucleotide exchange factor Dbl following ACK1-dependent tyrosine phosphorylation.

Kato J, Kaziro Y, Satoh T

Signals triggered by diverse receptors modulate the activity of Rho family proteins, although the regulatory mechanism remains largely unknown. On the basis of their biochemical activity as guanine nucleotide exchange factors (GEFs), Dbl family proteins are believed to be implicated in the regulation of Rho family GTP-binding proteins in response to a variety of extracellular stimuli. Here we show that ... [more]

Biochem. Biophys. Res. Commun. Feb. 05, 2000; 268(1);141-7 [Pubmed: 10652228]

Throughput

  • Low Throughput

Curated By

  • BioGRID