An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cutting edge: integration of human T lymphocyte cytoskeleton by the cytolinker plectin.

Brown MJ, Hallam JA, Liu Y, Yamada KM, Shaw S

Chemokine-induced polarization of lymphocytes involves the rapid collapse of vimentin intermediate filaments (IFs) into an aggregate within the uropod. Little is known about the interactions of lymphocyte vimentin with other cytoskeletal elements. We demonstrate that human peripheral blood T lymphocytes express plectin, an IF-binding, cytoskeletal cross-linking protein. Plectin associates with a complex of structural proteins including vimentin, actin, fodrin, moesin, ... [more]

J. Immunol. Jul. 15, 2001; 167(2);641-5 [Pubmed: 11441066]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LMNB1 SPTAN1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2022)	High	-	BioGRID	3439690

Curated By

BioGRID

Interaction Summary

SPTAN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

LMNB1