An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

GRASP-1: a neuronal RasGEF associated with the AMPA receptor/GRIP complex.

Ye B, Liao D, Zhang X, Zhang P, Dong H, Huganir RL

The PDZ domain-containing proteins, such as PSD-95 and GRIP, have been suggested to be involved in the targeting of glutamate receptors, a process that plays a critical role in the efficiency of synaptic transmission and plasticity. To address the molecular mechanisms underlying AMPA receptor synaptic localization, we have identified several GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within ... [more]

Neuron Jun. 01, 2000; 26(3);603-17 [Pubmed: 10896157]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GRIP1 GRIPAP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Ye B (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GRIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [NAS]beta-catenin binding [TAS]glucocorticoid receptor binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]receptor signaling complex scaffold activity [NAS]transcription coactivator activity [NAS]

Gene Ontology Cellular Component

GRIPAP1