An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Small G protein Ral and its downstream molecules regulate endocytosis of EGF and insulin receptors.

Nakashima S, Morinaka K, Koyama S, Ikeda M, Kishida M, Okawa K, Iwamatsu A, Kishida S, Kikuchi A

The involvement of Ral and its downstream molecules in receptor-mediated endocytosis was examined. Expression of either RalG23V or RalS28N, which are known to be constitutively active and dominantnegative forms, respectively, in A431 cells blocked internalization of epidermal growth factor (EGF). Stable expression of RalG23V or RalS28N in CHO-IR cells also inhibited internalization of insulin. Internalization of EGF and insulin was ... [more]

EMBO J. Jul. 01, 1999; 18(13);3629-42 [Pubmed: 10393179]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EPS15 REPS2	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Nakashima S (1999)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EPS15

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

REPS2