SYNE1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EMD
Gene Ontology Biological Process
- cellular response to growth factor stimulus [IMP]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- mitotic nuclear envelope reassembly [TAS]
- muscle contraction [TAS]
- muscle organ development [TAS]
- negative regulation of catenin import into nucleus [IMP]
- negative regulation of fibroblast proliferation [IMP]
- positive regulation of protein export from nucleus [IMP]
- regulation of canonical Wnt signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Nesprin-1alpha self-associates and binds directly to emerin and lamin A in vitro.
Nesprin-1alpha is a spectrin repeat (SR)-containing, transmembrane protein of the inner nuclear membrane, and is highly expressed in muscle cells. A yeast two-hybrid screen for nesprin-1alpha-interacting proteins showed that nesprin-1alpha interacted with itself. Blot overlay experiments revealed that nesprin-1alpha's third SR binds the fifth SR. The carboxy-terminal half of nesprin-1alpha directly bound lamin A, a nuclear intermediate filament protein. Biochemical ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EMD SYNE1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 55 | BioGRID | 2987596 |
Curated By
- BioGRID