BAIT

ASAP1

AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P

ArfGAP with SH3 domain, ankyrin repeat and PH domain 1

GO Process (1)

GO Function (1)

GO Component (0)

Gene Ontology Biological Process

cilium morphogenesis [IMP]

Gene Ontology Molecular Function

protein binding [IPI]

CRISPR Database HGNC Alliance of Genome Resources VEGA OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

REPS2

POB1, RP23-436I3.2

RALBP1 associated Eps domain containing protein 2

GO Process (0)

GO Function (0)

GO Component (0)

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction of POB1, a downstream molecule of small G protein Ral, with PAG2, a paxillin-binding protein, is involved in cell migration.

Oshiro T, Koyama S, Sugiyama S, Kondo A, Onodera Y, Asahara T, Sabe H, Kikuchi A

POB1 was previously identified as a RalBP1-binding protein. POB1 and RalBP1 function downstream of small G protein Ral and regulate receptor-mediated endocytosis. To look for additional functions of POB1, we screened for POB1-binding proteins using a yeast two-hybrid method and found that POB1 interacts with mouse ASAP1, which is a human PAG2 homolog. PAG2 is a paxillin-associated protein with ADP-ribosylation ... [more]

J. Biol. Chem. Oct. 11, 2002; 277(41);38618-26 [Pubmed: 12149250]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
REPS2 ASAP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Oshiro T (2002)	Low	-	BioGRID	-
ASAP1 REPS2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Oshiro T (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary