BAIT

HLA-C

D6S204, HLA-JY3, HLC-C, PSORS1, XXbac-BCX101P6.2
major histocompatibility complex, class I, C
Homo sapiens
PREY

HSPA5

BIP, GRP78, HEL-S-89n, MIF2
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Biogenesis of MHC class I antigens: involvement of multiple chaperone molecules.

Kahn-Perles B, Salamero J, Jouans O

To analyze the early events occurring during the folding and assembly of major histocompatibility complex class I antigens, we used a panel of P815 mouse mastocytoma transfectants expressing wild-type or mutant human leukocyte antigen (HLA)-Cw3 proteins. We observed that newly synthesized unassembled HLA-Cw3 heavy chains (Cw3 alpha) specifically associate with three major long-lived proteins denoted p105, p88 and p78, according ... [more]

Eur. J. Cell Biol. Jun. 01, 1994; 64(1);176-85 [Pubmed: 7957306]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HLA-C HSPA5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.8646BioGRID
3187928

Curated By

  • BioGRID